PathoPlant TU-Logo
Quality QU000292
 
General Information
Acc No QU000292
Type reaction
Negative false
Manual Score 1
Calculated Score 1
Description AtHIR1 was specifically tested for complex formation with RPS2. Pulldown with AtHIR1-Myc and RPS2-HPB
Comment Pulldown of AtHIR1-Myc was only achieved by co-expression of RPS2-HPB
Creation Oct 10 2011 02:34:08:983PM
Creator pb
Last Update Oct 19 2011 11:13:01:000AM
Last Updater pb
Reaction
Acc No RE000163
Name RPS2(A.t.) -- HIR1(A.t.)
Material
Name transgenic plant, heterologous
Fullname transgenic plant transformed with heterologous gene
Method
Name affinity precipitation
Fullname affinity precipitation in solution or batch
Description e.g. with sepharose or magneto beads, immunoprecipitation
Reference
Ref. 1   Acc No  RF000492
  Authors  Qi Y, Tsuda K, Nguyen le V, Wang X, Lin J, Murphy AS, Glazebrook J, Thordal-Christensen H, Katagiri F.
  Title  Physical Association of Arabidopsis Hypersensitive Induced Reaction Proteins (HIRs) with the Immune Receptor RPS2.
  Source  J Biol Chem 286: 31297-307 (2011)

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