PathoPlant TU-Logo
Reference RF000354
General Information
Acc No RF000354
Journal DNA Res
Authors Ichimura K, Mizoguchi T, Hayashida N, Seki M, Shinozaki K.
Title Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana.
Volume 5
Issue 6
Page 341-8
Year 1998
Abstract We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli. Affinity purified GST-ATMKK3 and GST-ATMKK4 proteins contained phosphorylation activity, which shows that both the ATMKK3 and ATMKK4 genes encode functional protein kinases. Northern blot analysis revealed that the ATMKK3 gene expressed in all the organs. The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.
PMID 10048483
Corresp Author Shinozaki K
Creation Jan 7 2010 03:31:59:967PM
Creator mr
Last Update Jan 7 2010 03:36:01:000PM
Last Updater mr
Database  Link 
PubMed  10048483

    printer-friendly version