Quality QU000614
 
General Information
Acc No QU000614
Type reaction
Negative false
Calculated Score 5
Comment Equal amount of GST and GST-ECIP1 fusion proteins was incubated with in vitro-translated 35S-Met-labelled EIN2-CD. clear signal was observed in GST-ECIP1 interacted proteins.
Creation Mar 20 2012 09:24:33:933AM
Creator pb
Last Update Mar 20 2012 09:34:31:000AM
Last Updater pb
Reaction
Acc No RE000356
Name ECIP1(A.t.)--EIN2(A.t.)
Material
Name recombinant protein from prokaryot
Fullname recombinant protein expressed in a prokaryotic organism
Description e.g. recombinant protein from E. coli, B. subtilis
Method
Name Pulldown assay
Fullname Pulldown assay
Description a method to analyze in vitro protein-protein interaction
Reference
Ref. 1   Acc No  RF000615
  Authors  Lei G, Shen M, Li ZG, Zhang B, Duan KX, Wang N, Cao YR, Zhang WK, Ma B, Ling HQ, Chen SY, Zhang JS.
  Title  EIN2 regulates salt stress response and interacts with a MA3 domain-containing protein ECIP1 in Arabidopsis.
  Source  Plant Cell Environ 34: 1678-92 (2011)