Reference RF000372
General Information
Acc No RF000372
Journal  Please select
Authors Gao M, Liu J, Bi D, Zhang Z, Cheng F, Chen S, Zhang Y.
Title MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plants.
Volume 18
Issue 12
Page 1190-8.
Year 2008
Abstract Mitogen-activated protein kinase (MAPK) cascades play important roles in regulating plant innate immune responses. In a genetic screen to search for mutants with constitutive defense responses, we identified multiple alleles of mpk4 and mekk1 that exhibit cell death and constitutive defense responses. Bimolecular fluorescence complementation (BiFC) analysis showed that both MPK4 and MEKK1 interact with MKK1 and MKK2, two closely related MAPK kinases. mkk1 and mkk2 single mutant plants do not have obvious mutant phenotypes. To test whether MKK1 and MKK2 function redundantly, mkk1 mkk2 double mutants were generated. The mkk1 mkk2 double mutant plants die at seedling stage and the seedling-lethality phenotype is temperature-dependent. Similar to the mpk4 and mekk1 mutants, the mkk1 mkk2 double mutant seedlings accumulate high levels of H2O2, display spontaneous cell death, constitutively express Pathogenesis Related (PR) genes and exhibit pathogen resistance. In addition, activation of MPK4 by flg22 is impaired in the mkk1 mkk2 double mutants, suggesting that MKK1 and MKK2 function together with MPK4 and MEKK1 in a MAP kinase cascade to negatively regulate innate immune responses in plants.
PMID 18982020
Corresp Author Gao M, Liu J, Bi D, Zhang Z, Cheng F, Chen S, Zhang Y.
Corresp Email College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Creation Nov 8 2010 10:35:41:293AM
Creator sp
Last Update Nov 9 2010 01:03:54:000PM
Last Updater sp
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PubMed  18982020