Reference RF000454
General Information
Acc No RF000454
Journal J Biol Chem
Authors Gao Z, Wen CK, Binder BM, Chen YF, Chang J, Chiang YH, Kerris RJ 3rd, Chang C, Schaller GE.
Title Heteromeric interactions among ethylene receptors mediate signaling in Arabidopsis.
Volume 283
Issue 35
Page 23801-10
Year 2008
Abstract The gaseous hormone ethylene is perceived in Arabidopsis by a five member receptor family that consists of the subfamily 1 receptors ETR1 and ERS1 and the subfamily 2 receptors ETR2, ERS2, and EIN4. Previous work has demonstrated that the basic functional unit for the ethylene receptor, ETR1, is a disulfide-linked homodimer. We demonstrate here that ethylene receptors isolated from Arabidopsis also interact with each other through noncovalent interactions. Evidence that ETR1 associates with other ethylene receptors was obtained by co-purification of ETR1 with tagged versions of ERS1, ETR2, ERS2, and EIN4 from Arabidopsis membrane extracts. ETR1 preferentially associated with the subfamily 2 receptors compared with the subfamily 1 receptor ERS1, but ethylene treatment affected the interactions and relative composition of the receptor complexes. When transgenically expressed in yeast, ETR1 and ERS2 can form disulfide-linked heterodimers. In plant extracts, however, the association of ETR1 and ERS2 can be largely disrupted by treatment with SDS, supporting a higher order noncovalent interaction between the receptors. Yeast two-hybrid analysis demonstrated that the receptor GAF domains are capable of mediating heteromeric receptor interactions. Kinetic analysis of ethylene-insensitive mutants of ETR1 is consistent with their dominance being due in part to an ability to associate with other ethylene receptors. These data suggest that the ethylene receptors exist in plants as clusters in a manner potentially analogous to that found with the histidine kinase-linked chemoreceptors of bacteria and that interactions among receptors contribute to ethylene signal output.
PMID 18577522
Corresp Author Gao Z, Wen CK, Binder BM, Chen YF, Chang J, Chiang YH, Kerris RJ 3rd, Chang C, Schaller GE.
Corresp Email Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA.
Creation Jan 20 2011 08:49:56:593AM
Creator sp
Last Update Jan 20 2011 08:50:53:000AM
Last Updater sp
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PubMed  18577522