PathoPlant TU-Logo
Quality QU000444
 
General Information
Acc No QU000444
Type reaction
Negative false
Calculated Score 2
Comment Coexpression of cLUC-JAZ8 and nLUC-MYB24 in N.bethamiana leaves leads to restoration of the LUC activity
Creation Dec 30 2011 11:30:07:670AM
Creator pb
Last Update Dec 30 2011 11:30:26:000AM
Last Updater pb
Reaction
Acc No RE000252
Name MYB24(A.t.)->JAZ8(A.t.)
Material
Name transfected tissue, heterologous
Fullname transiently transfected tissue with heterologous gene
Method
Name LCI
Fullname LUC complementation imaging Assay
Description Method to check protein-protein interaction by dividing the LUC reporter gene into a N-terminal and a C-terminal part. By fusing each part to a different protein of interest the LUC-activity can be restored when those proteins interact physically
Reference
Ref. 1   Acc No  RF000530
  Authors  Song S, Qi T, Huang H, Ren Q, Wu D, Chang C, Peng W, Liu Y, Peng J, Xie D.
  Title  The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis.
  Source  Plant Cell 23: 1000-13 (2011)

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